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M Graca N. Almeida-Porada MD, PhD

TitleProfessor
InstitutionWake Forest School of Medicine
DepartmentWake Forest Institute for Regenerative Medicine
Address
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    Other Positions
    TitleProfessor
    InstitutionWake Forest School of Medicine
    DepartmentBiomedical Engineering

    TitleProfessor
    InstitutionWake Forest School of Medicine
    DepartmentInternal Medicine, Hematology & Oncology


    Collapse Research 
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    R01HL070566     (ALMEIDA-PORADA, GRACA DUARTE)Sep 20, 2001 - Aug 31, 2006
    NIH/NHLBI
    Evaluation of Stem Cell Plasticity In Vivo
    Role: Principal Investigator
    R01HL073737     (ALMEIDA-PORADA, GRACA DUARTE)Jul 1, 2003 - Jun 30, 2009
    NIH/NHLBI
    In Utero Stem Cell Transplantation for Hemophilia A
    Role: Principal Investigator
    R01HL097623     (ALMEIDA-PORADA, GRACA DUARTE)Aug 28, 2009 - Jul 31, 2013
    NIH/NHLBI
    Functional Analysis of the Hematopoietic Niche during Ontogeny
    Role: Principal Investigator
    R21HL117704     (ALMEIDA-PORADA, GRACA DUARTE)Jul 19, 2013 - Jun 30, 2016
    NIH
    Cord Blood Expansion Inside a Bioengineered Liver
    Role: Principal Investigator
    R01HL130856     (ALMEIDA-PORADA, GRACA DUARTE)Sep 1, 2016 - May 31, 2021
    NIH
    Postnatal Cell-Based Therapies for Hemophilia A
    Role: Principal Investigator
    R01HL135853     (ALMEIDA-PORADA, GRACA DUARTE)Jul 14, 2017 - Jun 30, 2021
    NIH
    Prenatal Cell and Gene Therapy for Hemophilia A
    Role: Principal Investigator
    U01HL148681     (ALMEIDA-PORADA, GRACA DUARTE)Jul 1, 2019 - Jun 30, 2021
    NIH
    cGMP Manufacture Of FVIII-Expressing Placental Cells For Hemophilia A
    Role: Principal Investigator
    R01HL161290     (ALMEIDA-PORADA, GRACA DUARTE ;PORADA, CHRISTOPHER D)Sep 15, 2022 - Jun 30, 2026
    NIH
    Defining the therapeutic efficacy, tolerogenic potential, and genotoxicity of liver-targeted AAV gene therapy for hemophilia A
    Role: Principal Investigator
    1R01HL166462-01     (ALMEIDA-PORADA, GRACA DUARTE)Feb 15, 2023 - Jan 31, 2027
    National Heart and Lung Blood Institute
    Using human liver tissue equivalents to optimize AAV-mediated GT and better define age-related clinical risks
    Role: Principal Investigator
    1R01HL165247-01A1     (ALMEIDA-PORADA, GRACA DUARTE)Jul 1, 2023 - Jun 30, 2027
    National Heart and Lung Blood Institute
    Targeted conditioning to maximize prenatal HSC engraftment for SCD
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Rodriguez M, Trevisan B, Ramamurthy RM, George SK, Diaz J, Alexander J, Meares D, Schwahn DJ, Quilici DR, Figueroa J, Gautreaux M, Farland A, Atala A, Doering CB, Spencer HT, Porada CD, Almeida-Porada G. Transplanting FVIII/ET3-secreting cells in fetal sheep increases FVIII levels long-term without inducing immunity or toxicity. Nat Commun. 2023 Jul 14; 14(1):4206. PMID: 37452013.
      Citations:    
    2. Pham QDM, Thomson SM, Schaible BN, Mills KD, Atala A, Porada CD, Almeida-Porada G. Acceptability of prenatal diagnosis and prenatal treatment of haemophilia using cell and gene therapies within US haemophilia community. Haemophilia. 2023 Jul; 29(4):1024-1031. PMID: 37228173.
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    3. Ramamurthy RM, Rodriguez M, Ainsworth HC, Shields J, Meares D, Bishop C, Farland A, Langefeld CD, Atala A, Doering CB, Spencer HT, Porada CD, Almeida-Porada G. Comparison of different gene addition strategies to modify placental derived-mesenchymal stromal cells to produce FVIII. Front Immunol. 2022; 13:954984. PMID: 36591257.
      Citations:    
    4. Trevisan B, Rodriguez M, Medder H, Lankford S, Combs R, Owen J, Atala A, Porada CD, Almeida-Porada G. Autologous bone marrow-derived MSCs engineered to express oFVIII-FLAG engraft in adult sheep and produce an effective increase in plasma FVIII levels. Front Immunol. 2022; 13:1070476. PMID: 36532079.
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    5. Evans AC, Martin KA, Saxena M, Bicher S, Wheeler E, Cordova EJ, Porada CD, Almeida-Porada G, Kato TA, Wilson PF, Coleman MA. Curcumin Nanodiscs Improve Solubility and Serve as Radiological Protectants against Ionizing Radiation Exposures in a Cell-Cycle Dependent Manner. Nanomaterials (Basel). 2022 Oct 15; 12(20). PMID: 36296810.
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    6. Ramamurthy RM, Atala A, Porada CD, Almeida-Porada G. Organoids and microphysiological systems: Promising models for accelerating AAV gene therapy studies. Front Immunol. 2022; 13:1011143. PMID: 36225917.
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    7. de Coppi P, Loukogeorgakis S, Götherström C, David AL, Almeida-Porada G, Chan JKY, Deprest J, Wong KKY, Tam PKH. Regenerative medicine: prenatal approaches. Lancet Child Adolesc Health. 2022 Sep; 6(9):643-653. PMID: 35963269.
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    8. Herzeg A, Almeida-Porada G, Charo RA, David AL, Gonzalez-Velez J, Gupta N, Lapteva L, Lianoglou B, Peranteau W, Porada C, Sanders SJ, Sparks TN, Stitelman DH, Struble E, Sumner CJ, MacKenzie TC. Prenatal Somatic Cell Gene Therapies: Charting a Path Toward Clinical Applications (Proceedings of the CERSI-FDA Meeting). J Clin Pharmacol. 2022 Sep; 62 Suppl 1(Suppl 1):S36-S52. PMID: 36106778.
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    9. Wu R, Soland M, Liu G, Shi Y, Zhang C, Tang Y, Almeida-Porada G, Zhang Y. Functional characterization of the immunomodulatory properties of human urine-derived stem cells. Transl Androl Urol. 2021 Sep; 10(9):3566-3578. PMID: 34733653.
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    10. Stem C, Rodman C, Ramamurthy RM, George S, Meares D, Farland A, Atala A, Doering CB, Spencer HT, Porada CD, Almeida-Porada G. Investigating Optimal Autologous Cellular Platforms for Prenatal or Perinatal Factor VIII Delivery to Treat Hemophilia A. Front Cell Dev Biol. 2021; 9:678117. PMID: 34447745.
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    11. Almeida-Porada G. A new "FIX" for hemophilia B gene therapy. Blood. 2021 May 27; 137(21):2860-2861. PMID: 34042982.
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    12. Trevisan B, Morsi A, Aleman J, Rodriguez M, Shields J, Meares D, Farland AM, Doering CB, Spencer HT, Atala A, Skardal A, Porada CD, Almeida-Porada G. Effects of Shear Stress on Production of FVIII and vWF in a Cell-Based Therapeutic for Hemophilia A. Front Bioeng Biotechnol. 2021; 9:639070. PMID: 33732691.
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    13. Sagar R, Almeida-Porada G, Blakemore K, Chan JKY, Choolani M, Götherström C, Jaulent A, MacKenzie TC, Mattar C, Porada CD, Peranteau WH, Schneider H, Shaw SW, Waddington SN, Westgren M, David AL. Fetal and Maternal Safety Considerations for In Utero Therapy Clinical Trials: iFeTiS Consensus Statement. Mol Ther. 2020 11 04; 28(11):2316-2319. PMID: 33069884.
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    14. O'Connell AE, Guseh S, Lapteva L, Cummings CL, Wilkins-Haug L, Chan J, Peranteau WH, Almeida-Porada G, Kourembanas S. Gene and Stem Cell Therapies for Fetal Care: A Review. JAMA Pediatr. 2020 Oct 01; 174(10):985-991. PMID: 32597943.
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    15. Trevisan BM, Porada CD, Atala A, Almeida-Porada G. Microfluidic devices for studying coagulation biology. Semin Cell Dev Biol. 2021 Apr; 112:1-7. PMID: 32563678.
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    16. El-Akabawy N, Rodriguez M, Ramamurthy R, Rabah A, Trevisan B, Morsi A, George S, Shields J, Meares D, Farland A, Atala A, Doering CB, Spencer HT, Porada CD, Almeida-Porada G. Defining the Optimal FVIII Transgene for Placental Cell-Based Gene Therapy to Treat Hemophilia A. Mol Ther Methods Clin Dev. 2020 Jun 12; 17:465-477. PMID: 32258210.
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    17. Chiloff DM, de Almeida DC, Dalboni MA, Canziani ME, George SK, Morsi AM, El-Akabawy N, Porada CD, Durao MS, Zarjou A, Almeida-Porada G, Goes MA. Soluble Fas affects erythropoiesis in vitro and acts as a potential predictor of erythropoiesis-stimulating agent therapy in patients with chronic kidney disease. Am J Physiol Renal Physiol. 2020 04 01; 318(4):F861-F869. PMID: 32003597.
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    18. Almeida-Porada G, Atala AJ, Porada CD. Therapeutic Mesenchymal Stromal Cells for Immunotherapy and for Gene and Drug Delivery. Mol Ther Methods Clin Dev. 2020 Mar 13; 16:204-224. PMID: 32071924.
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    19. Rodriguez M, Porada CD, Almeida-Porada G. Mechanistic Insights into Factor VIII Immune Tolerance Induction via Prenatal Cell Therapy in Hemophilia A. Curr Stem Cell Rep. 2019 Dec; 5(4):145-161. PMID: 32351874.
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    20. Aleman J, George SK, Herberg S, Devarasetty M, Porada CD, Skardal A, Almeida-Porada G. Deconstructed Microfluidic Bone Marrow On-A-Chip to Study Normal and Malignant Hemopoietic Cell-Niche Interactions. Small. 2019 10; 15(43):e1902971. PMID: 31464364.
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    21. Cary L, Noutai D, Salber R, Fadiyimu O, Gross A, Almeida-Porada G, Kidane Y, Whitnall M. Bone Marrow Endothelial Cells Influence Function and Phenotype of Hematopoietic Stem and Progenitor Cells after Mixed Neutron/Gamma Radiation. Int J Mol Sci. 2019 Apr 11; 20(7). PMID: 30978983.
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    22. Almeida-Porada G, Waddington SN, Chan JKY, Peranteau WH, MacKenzie T, Porada CD. In Utero Gene Therapy Consensus Statement from the IFeTIS. Mol Ther. 2019 04 10; 27(4):705-707. PMID: 30837116.
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    23. Ferreira JR, Teixeira GQ, Santos SG, Barbosa MA, Almeida-Porada G, Gonçalves RM. Mesenchymal Stromal Cell Secretome: Influencing Therapeutic Potential by Cellular Pre-conditioning. Front Immunol. 2018; 9:2837. PMID: 30564236.
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    24. Selicean SE, Tomuleasa C, Grewal R, Almeida-Porada G, Berindan-Neagoe I. Mesenchymal stem cells in myeloproliferative disorders - focus on primary myelofibrosis. Leuk Lymphoma. 2018 Oct 02; 1-10. PMID: 30277128.
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    25. Leitão L, Alves CJ, Alencastre IS, Sousa DM, Neto E, Conceição F, Leitão C, Aguiar P, Almeida-Porada G, Lamghari M. Bone marrow cell response after injury and during early stage of regeneration is independent of the tissue-of-injury in 2 injury models. FASEB J. 2019 Jan; 33(1):857-872. PMID: 30044924.
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    26. Low EK, Brudvik E, Kuhlman B, Wilson PF, Almeida-Porada G, Porada CD. Microgravity Impairs DNA Damage Repair in Human Hematopoietic Stem/Progenitor Cells and Inhibits Their Differentiation into Dendritic Cells. Stem Cells Dev. 2018 09 15; 27(18):1257-1267. PMID: 29901426.
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    27. Mokhtari S, Colletti E, Yin W, Sanada C, Lamar Z, Simmons PJ, Walker S, Bishop C, Atala A, Zanjani ED, Porada CD, Almeida-Porada G. Correction: A human bone marrow mesodermal-derived cell population with hemogenic potential. Leukemia. 2018 07; 32(7):1670. PMID: 29891936.
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    28. Ross CL, Pettenati MJ, Procita J, Cathey L, George SK, Almeida-Porada G. Evaluation of Cytotoxic and Genotoxic Effects of Extremely Low-frequency Electromagnetic Field on Mesenchymal Stromal Cells. Glob Adv Health Med. 2018; 7:2164956118777472. PMID: 29796339.
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    29. Almeida-Porada G, Rodman C, Kuhlman B, Brudvik E, Moon J, George S, Guida P, Sajuthi SP, Langefeld CD, Walker SJ, Wilson PF, Porada CD. Exposure of the Bone Marrow Microenvironment to Simulated Solar and Galactic Cosmic Radiation Induces Biological Bystander Effects on Human Hematopoiesis. Stem Cells Dev. 2018 09 15; 27(18):1237-1256. PMID: 29698131.
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    30. Mokhtari S, Baptista PM, Vyas DA, Freeman CJ, Moran E, Brovold M, Llamazares GA, Lamar Z, Porada CD, Soker S, Almeida-Porada G. Evaluating Interaction of Cord Blood Hematopoietic Stem/Progenitor Cells with Functionally Integrated Three-Dimensional Microenvironments. Stem Cells Transl Med. 2018 03; 7(3):271-282. PMID: 29473346.
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    31. Mokhtari S, Colletti E, Yin W, Sanada C, Lamar Z, Simmons PJ, Walker S, Bishop C, Atala A, Zanjani ED, Porada CD, Almeida-Porada G. A human bone marrow mesodermal-derived cell population with hemogenic potential. Leukemia. 2018 07; 32(7):1575-1586. PMID: 29467489.
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    32. Qi B, Crawford AJ, Wojtynek NE, Holmes MB, Souchek JJ, Almeida-Porada G, Ly QP, Cohen SM, Hollingsworth MA, Mohs AM. Indocyanine green loaded hyaluronan-derived nanoparticles for fluorescence-enhanced surgical imaging of pancreatic cancer. Nanomedicine. 2018 04; 14(3):769-780. PMID: 29325740.
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    33. Rodman C, Almeida-Porada G, George SK, Moon J, Soker S, Pardee T, Beaty M, Guida P, Sajuthi SP, Langefeld CD, Walker SJ, Wilson PF, Porada CD. In vitro and in vivo assessment of direct effects of simulated solar and galactic cosmic radiation on human hematopoietic stem/progenitor cells. Leukemia. 2017 06; 31(6):1398-1407. PMID: 27881872.
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    34. Mokhtari S, Colletti EJ, Atala A, Zanjani ED, Porada CD, Almeida-Porada G. Boosting Hematopoietic Engraftment after in Utero Transplantation through Vascular Niche Manipulation. Stem Cell Reports. 2016 06 14; 6(6):957-969. PMID: 27304918.
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    35. Almeida-Porada G, Atala A, Porada CD. In utero stem cell transplantation and gene therapy: rationale, history, and recent advances toward clinical application. Mol Ther Methods Clin Dev. 2016; 5:16020. PMID: 27069953.
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    36. Sunwook K, Nussbaum MA, Quandt SA, Laurienti PJ, Arcury TA. Effects of Lifetime Occupational Pesticide Exposure on Postural Control Among Farmworkers and Non-Farmworkers. J Occup Environ Med. 2016 Feb; 58(2):133-9. PMID: 30886614.
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    37. Porada CD, Atala AJ, Almeida-Porada G. The hematopoietic system in the context of regenerative medicine. Methods. 2016 Apr 15; 99:44-61. PMID: 26319943.
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    38. Ross CL, Siriwardane M, Almeida-Porada G, Porada CD, Brink P, Christ GJ, Harrison BS. The effect of low-frequency electromagnetic field on human bone marrow stem/progenitor cell differentiation. Stem Cell Res. 2015 Jul; 15(1):96-108. PMID: 26042793.
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    39. MacKenzie TC, David AL, Flake AW, Almeida-Porada G. Consensus statement from the first international conference for in utero stem cell transplantation and gene therapy. Front Pharmacol. 2015; 6:15. PMID: 25713535.
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    40. Shaw SW, Blundell MP, Pipino C, Shangaris P, Maghsoudlou P, Ramachandra DL, Georgiades F, Boyd M, Thrasher AJ, Porada CD, Almeida-Porada G, Cheng PJ, David AL, de Coppi P. Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation. Stem Cells. 2015 Jan; 33(1):122-32. PMID: 25186828.
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    41. Porada CD, Rodman C, Ignacio G, Atala A, Almeida-Porada G. Hemophilia A: an ideal disease to correct in utero. Front Pharmacol. 2014; 5:276. PMID: 25566073.
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    42. Boura JS, Vance M, Yin W, Madeira C, Lobato da Silva C, Porada CD, Almeida-Porada G. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev. 2014 Sep; 2014(1). PMID: 25279386.
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    43. Jeanblanc C, Goodrich AD, Colletti E, Mokhtari S, Porada CD, Zanjani ED, Almeida-Porada G. Temporal definition of haematopoietic stem cell niches in a large animal model of in utero stem cell transplantation. Br J Haematol. 2014 Jul; 166(2):268-78. PMID: 24673111.
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    44. Soland MA, Keyes LR, Bayne R, Moon J, Porada CD, St Jeor S, Almeida-Porada G. Perivascular stromal cells as a potential reservoir of human cytomegalovirus. Am J Transplant. 2014 Apr; 14(4):820-30. PMID: 24592822.
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    45. Porada CD, Stem C, Almeida-Porada G. Gene therapy: the promise of a permanent cure. N C Med J. 2013 Nov-Dec; 74(6):526-9. PMID: 24316783.
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    46. Tellez J, Van Vliet K, Tseng YS, Finn JD, Tschernia N, Almeida-Porada G, Arruda VR, Agbandje-McKenna M, Porada CD. Characterization of naturally-occurring humoral immunity to AAV in sheep. PLoS One. 2013; 8(9):e75142. PMID: 24086458.
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    47. Almeida-Porada G, Soland M, Boura J, Porada CD. Regenerative medicine: prospects for the treatment of inflammatory bowel disease. Regen Med. 2013 Sep; 8(5):631-44. PMID: 23998755.
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    48. Sanada C, Kuo CJ, Colletti EJ, Soland M, Mokhtari S, Knovich MA, Owen J, Zanjani ED, Porada CD, Almeida-Porada G. Mesenchymal stem cells contribute to endogenous FVIII:c production. J Cell Physiol. 2013 May; 228(5):1010-6. PMID: 23042590.
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    49. Soland MA, Bego M, Colletti E, Zanjani ED, St Jeor S, Porada CD, Almeida-Porada G. Mesenchymal stem cells engineered to inhibit complement-mediated damage. PLoS One. 2013; 8(3):e60461. PMID: 23555976.
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    50. Colletti E, El Shabrawy D, Soland M, Yamagami T, Mokhtari S, Osborne C, Schlauch K, Zanjani ED, Porada CD, Almeida-Porada G. EphB2 isolates a human marrow stromal cell subpopulation with enhanced ability to contribute to the resident intestinal cellular pool. FASEB J. 2013 Jun; 27(6):2111-21. PMID: 23413357.
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    51. Keyes LR, Hargett D, Soland M, Bego MG, Rossetto CC, Almeida-Porada G, St Jeor S. HCMV protein LUNA is required for viral reactivation from latently infected primary CD14? cells. PLoS One. 2012; 7(12):e52827. PMID: 23300789.
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    52. Zakas PM, Gangadharan B, Almeida-Porada G, Porada CD, Spencer HT, Doering CB. Development and characterization of recombinant ovine coagulation factor VIII. PLoS One. 2012; 7(11):e49481. PMID: 23152911.
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    53. Wood JA, Colletti E, Mead LE, Ingram D, Porada CD, Zanjani ED, Yoder MC, Almeida-Porada G. Distinct contribution of human cord blood-derived endothelial colony forming cells to liver and gut in a fetal sheep model. Hepatology. 2012 Sep; 56(3):1086-96. PMID: 22488442.
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    54. Soland MA, Bego MG, Colletti E, Porada CD, Zanjani ED, St Jeor S, Almeida-Porada G. Modulation of human mesenchymal stem cell immunogenicity through forced expression of human cytomegalovirus us proteins. PLoS One. 2012; 7(5):e36163. PMID: 22666319.
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    55. Porada CD, Almeida-Porada G. Treatment of Hemophilia A in Utero and Postnatally using Sheep as a Model for Cell and Gene Delivery. J Genet Syndr Gene Ther. 2012 May 25; S1. PMID: 23264887.
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    56. Ozturk F, Park PJ, Tellez J, Colletti E, Eiden MV, Almeida-Porada G, Porada CD. Expression levels of the PiT-2 receptor explain, in part, the gestational age-dependent alterations in transduction efficiency after in utero retroviral-mediated gene transfer. J Gene Med. 2012 Mar; 14(3):169-81. PMID: 22262359.
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    57. Porada CD, Sanada C, Kuo CJ, Colletti E, Mandeville W, Hasenau J, Zanjani ED, Moot R, Doering C, Spencer HT, Almeida-Porada G. Phenotypic correction of hemophilia A in sheep by postnatal intraperitoneal transplantation of FVIII-expressing MSC. Exp Hematol. 2011 Dec; 39(12):1124-1135.e4. PMID: 21906573.
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    58. Andrade PZ, da Silva CL, dos Santos F, Almeida-Porada G, Cabral JM. Initial CD34+ cell-enrichment of cord blood determines hematopoietic stem/progenitor cell yield upon ex vivo expansion. J Cell Biochem. 2011 Jul; 112(7):1822-31. PMID: 21400571.
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    59. Mishra R, Vijayan K, Colletti EJ, Harrington DA, Matthiesen TS, Simpson D, Goh SK, Walker BL, Almeida-Porada G, Wang D, Backer CL, Dudley SC, Wold LE, Kaushal S. Characterization and functionality of cardiac progenitor cells in congenital heart patients. Circulation. 2011 Feb 01; 123(4):364-73. PMID: 21242485.
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    60. Almeida-Porada GD. Stem cell gene manipulation and delivery as systemic therapeutics. Adv Drug Deliv Rev. 2010 Sep 30; 62(12):1139-40. PMID: 20955742.
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    61. Porada CD, Almeida-Porada G. Mesenchymal stem cells as therapeutics and vehicles for gene and drug delivery. Adv Drug Deliv Rev. 2010 Sep 30; 62(12):1156-66. PMID: 20828588.
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    62. Russo-Carbolante EM, Picanço-Castro V, Alves DC, Fernandes AC, Almeida-Porada G, Tonn T, Covas DT. Integration pattern of HIV-1 based lentiviral vector carrying recombinant coagulation factor VIII in Sk-Hep and 293T cells. Biotechnol Lett. 2011 Jan; 33(1):23-31. PMID: 20812025.
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    63. Gonçalves R, Martins MC, Oliveira MJ, Almeida-Porada G, Barbosa MA. Bioactivity of immobilized EGF on self-assembled monolayers: optimization of the immobilization process. J Biomed Mater Res A. 2010 Aug; 94(2):576-85. PMID: 20198690.
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    64. Andrade PZ, dos Santos F, Almeida-Porada G, da Silva CL, S Cabral JM. Systematic delineation of optimal cytokine concentrations to expand hematopoietic stem/progenitor cells in co-culture with mesenchymal stem cells. Mol Biosyst. 2010 Jul; 6(7):1207-15. PMID: 20424784.
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    65. Almeida-Porada G, Zanjani ED, Porada CD. Bone marrow stem cells and liver regeneration. Exp Hematol. 2010 Jul; 38(7):574-80. PMID: 20417684.
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    66. Goodrich AD, Ersek A, Varain NM, Groza D, Cenariu M, Thain DS, Almeida-Porada G, Porada CD, Zanjani ED. In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cells. Exp Hematol. 2010 Jun; 38(6):516-525.e4. PMID: 20227460.
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    67. Ersek A, Pixley JS, Goodrich AD, Porada CD, Almeida-Porada G, Thain DS, Zanjani ED. Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cells. Exp Hematol. 2010 Apr; 38(4):311-20. PMID: 20170708.
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    68. da Silva CL, Gonçalves R, dos Santos F, Andrade PZ, Almeida-Porada G, Cabral JM. Dynamic cell-cell interactions between cord blood haematopoietic progenitors and the cellular niche are essential for the expansion of CD34+, CD34+CD38- and early lymphoid CD7+ cells. J Tissue Eng Regen Med. 2010 Feb; 4(2):149-58. PMID: 19937912.
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    69. Porada CD, Sanada C, Long CR, Wood JA, Desai J, Frederick N, Millsap L, Bormann C, Menges SL, Hanna C, Flores-Foxworth G, Shin T, Westhusin ME, Liu W, Glimp H, Zanjani ED, Lozier JN, Pliska V, Stranzinger G, Joerg H, Kraemer DC, Almeida-Porada G. Clinical and molecular characterization of a re-established line of sheep exhibiting hemophilia A. J Thromb Haemost. 2010 Feb; 8(2):276-85. PMID: 19943872.
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    70. Gonçalves RM, Martins MC, Almeida-Porada G, Barbosa MA. Induction of notch signaling by immobilization of jagged-1 on self-assembled monolayers. Biomaterials. 2009 Dec; 30(36):6879-87. PMID: 19783294.
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    71. da Silva CL, Gonçalves R, Porada CD, Ascensão JL, Zanjani ED, Cabral JM, Almeida-Porada G. Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics. J Cell Physiol. 2009 Jul; 220(1):102-11. PMID: 19277981.
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    72. Park PJ, Colletti E, Ozturk F, Wood JA, Tellez J, Almeida-Porada G, Porada C. Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheep. Hum Gene Ther. 2009 Mar; 20(3):201-15. PMID: 19301473.
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    73. Yamagami T, Porada CD, Pardini RS, Zanjani ED, Almeida-Porada G. Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell line. . 2009 Feb; 8(4):331-7. PMID: 19197149.
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    74. Porada CD, Harrison-Findik DD, Sanada C, Valiente V, Thain D, Simmons PJ, Almeida-Porada G, Zanjani ED. Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells. Exp Hematol. 2008 Dec; 36(12):1739-49. PMID: 19007686.
      Citations:    
    75. Park PJ, Colletti E, Ozturk F, Wood JA, Tellez J, Almeida-Porada G, Porada C. Factors Determining the Risk of Inadvertent Retroviral Transduction of Male Germ Cells Following in Utero Gene Transfer in Sheep. Hum Gene Ther. 2008 Nov 26. PMID: 19035843.
      Citations:    
    76. Barrias CC, Martins MC, Almeida-Porada G, Barbosa MA, Granja PL. The correlation between the adsorption of adhesive proteins and cell behaviour on hydroxyl-methyl mixed self-assembled monolayers. Biomaterials. 2009 Jan; 30(3):307-16. PMID: 18952279.
      Citations:    
    77. Colletti EJ, Airey JA, Liu W, Simmons PJ, Zanjani ED, Porada CD, Almeida-Porada G. Generation of tissue-specific cells from MSC does not require fusion or donor-to-host mitochondrial/membrane transfer. Stem Cell Res. 2009 Mar; 2(2):125-38. PMID: 19383418.
      Citations:    
    78. Colletti E, Lindstedt S, Park PJ, Almeida-Porada G, Porada CD. Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction. Exp Hematol. 2008 Jul; 36(7):816-22. PMID: 18400364.
      Citations:    
    79. Frias AM, Porada CD, Crapnell KB, Cabral JM, Zanjani ED, Almeida-Porada G. Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion. Exp Hematol. 2008 Jan; 36(1):61-8. PMID: 18155552.
      Citations:    
    80. Chamberlain J, Yamagami T, Colletti E, Theise ND, Desai J, Frias A, Pixley J, Zanjani ED, Porada CD, Almeida-Porada G. Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep. Hepatology. 2007 Dec; 46(6):1935-45. PMID: 17705296.
      Citations:    
    81. Almeida-Porada G, Porada C, Gupta N, Torabi A, Thain D, Zanjani ED. The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential. Exp Hematol. 2007 Oct; 35(10):1594-600. PMID: 17889724.
      Citations:    
    82. Gonçalves R, Lobato da Silva C, Cabral JM, Zanjani ED, Almeida-Porada G. A Stro-1(+) human universal stromal feeder layer to expand/maintain human bone marrow hematopoietic stem/progenitor cells in a serum-free culture system. Exp Hematol. 2006 Oct; 34(10):1353-9. PMID: 16982328.
      Citations:    
    83. Colletti EJ, Almeida-Porada G, Chamberlain J, Zanjani ED, Airey JA. The time course of engraftment of human mesenchymal stem cells in fetal heart demonstrates that Purkinje fiber aggregates derive from a single cell and not multi-cell homing. Exp Hematol. 2006 Jul; 34(7):926-33. PMID: 16797420.
      Citations:    
    84. Horváth VJ, Vittal H, Lörincz A, Chen H, Almeida-Porada G, Redelman D, Ordög T. Reduced stem cell factor links smooth myopathy and loss of interstitial cells of cajal in murine diabetic gastroparesis. Gastroenterology. 2006 Mar; 130(3):759-70. PMID: 16530517.
      Citations:    
    85. Gonçalves R, da Silva CL, Ferreira BS, Lemos MA, Lemos F, Almeida-Porada G, Cabral JM. Kinetic analysis of the ex vivo expansion of human hematopoietic stem/progenitor cells. Biotechnol Lett. 2006 Mar; 28(5):335-40. PMID: 16614921.
      Citations:    
    86. Porada CD, Park PJ, Tellez J, Ozturk F, Glimp HA, Almeida-Porada G, Zanjani ED. Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in utero. Mol Ther. 2005 Oct; 12(4):754-62. PMID: 16084775.
      Citations:    
    87. da Silva CL, Gonçalves R, Crapnell KB, Cabral JM, Zanjani ED, Almeida-Porada G. A human stromal-based serum-free culture system supports the ex vivo expansion/maintenance of bone marrow and cord blood hematopoietic stem/progenitor cells. Exp Hematol. 2005 Jul; 33(7):828-35. PMID: 15963859.
      Citations:    
    88. Almeida-Porada G, Crapnell K, Porada C, Benoit B, Nakauchi H, Quesenberry P, Zanjani ED. In vivo haematopoietic potential of human neural stem cells. Br J Haematol. 2005 Jul; 130(2):276-83. PMID: 16029457.
      Citations:    
    89. Porada CD, Park PJ, Almeida-Porada G, Liu W, Ozturk F, Glimp HA, Zanjani ED. Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer. Mol Ther. 2005 Feb; 11(2):284-93. PMID: 15668140.
      Citations:    
    90. Kögler G, Sensken S, Airey JA, Trapp T, Müschen M, Feldhahn N, Liedtke S, Sorg RV, Fischer J, Rosenbaum C, Greschat S, Knipper A, Bender J, Degistirici O, Gao J, Caplan AI, Colletti EJ, Almeida-Porada G, Müller HW, Zanjani E, Wernet P. A new human somatic stem cell from placental cord blood with intrinsic pluripotent differentiation potential. J Exp Med. 2004 Jul 19; 200(2):123-35. PMID: 15263023.
      Citations:    
    91. Almeida-Porada G, Porada CD, Chamberlain J, Torabi A, Zanjani ED. Formation of human hepatocytes by human hematopoietic stem cells in sheep. Blood. 2004 Oct 15; 104(8):2582-90. PMID: 15231580.
      Citations:    
    92. Porada GA, Porada C, Zanjani ED. The fetal sheep: a unique model system for assessing the full differentiative potential of human stem cells. Yonsei Med J. 2004 Jun 30; 45 Suppl:7-14. PMID: 15250043.
      Citations:    
    93. Airey JA, Almeida-Porada G, Colletti EJ, Porada CD, Chamberlain J, Movsesian M, Sutko JL, Zanjani ED. Human mesenchymal stem cells form Purkinje fibers in fetal sheep heart. Circulation. 2004 Mar 23; 109(11):1401-7. PMID: 15023887.
      Citations:    
    94. Crapnell KB, Almeida-Porada G, Khaiboullina S, St Jeor SC, Zanjani ED. Human haematopoietic stem cells that mediate long-term in vivo engraftment are not susceptible to infection by human cytomegalovirus. Br J Haematol. 2004 Mar; 124(5):676-84. PMID: 14871256.
      Citations:    
    95. Almeida-Porada G, Zanjani ED. A large animal noninjury model for study of human stem cell plasticity. Blood Cells Mol Dis. 2004 Jan-Feb; 32(1):77-81. PMID: 14757417.
      Citations:    
    96. Almeida-Porada G, Porada C, Zanjani ED. Plasticity of human stem cells in the fetal sheep model of human stem cell transplantation. Int J Hematol. 2004 Jan; 79(1):1-6. PMID: 14979471.
      Citations:    
    97. Porada CD, Park P, Almeida-Porada G, Zanjani ED. The sheep model of in utero gene therapy. Fetal Diagn Ther. 2004 Jan-Feb; 19(1):23-30. PMID: 14646413.
      Citations:    
    98. Ordög T, Redelman D, Miller LJ, Horváth VJ, Zhong Q, Almeida-Porada G, Zanjani ED, Horowitz B, Sanders KM. Purification of interstitial cells of Cajal by fluorescence-activated cell sorting. . 2004 Feb; 286(2):C448-56. PMID: 14534083.
      Citations:    
    99. Zanjani ED, Almeida-Porada G, Livingston AG, Zeng H, Ogawa M. Reversible expression of CD34 by adult human bone marrow long-term engrafting hematopoietic stem cells. Exp Hematol. 2003 May; 31(5):406-12. PMID: 12763139.
      Citations:    
    100. Cabrita GJ, Ferreira BS, da Silva CL, Gonçalves R, Almeida-Porada G, Cabral JM. Hematopoietic stem cells: from the bone to the bioreactor. Trends Biotechnol. 2003 May; 21(5):233-40. PMID: 12727385.
      Citations:    
    101. Eaves C, Jiang X, Eisterer W, Chalandon Y, Porada G, Zanjani E, Eaves A. New models to investigate mechanisms of disease genesis from primitive BCR-ABL(+) hematopoietic cells. Ann N Y Acad Sci. 2003 May; 996:1-9. PMID: 12799276.
      Citations:    
    102. da Silva CL, Gonçalves R, Lemos F, Lemos MA, Zanjani ED, Almeida-Porada G, Cabral JM. Modelling of ex vivo expansion/maintenance of hematopoietic stem cells. Bioprocess Biosyst Eng. 2003 Jul; 25(6):365-9. PMID: 13680337.
      Citations:    
    103. Almeida-Porada G, El Shabrawy D, Porada C, Zanjani ED. Differentiative potential of human metanephric mesenchymal cells. Exp Hematol. 2002 Dec; 30(12):1454-62. PMID: 12482508.
      Citations:    
    104. Barão I, Vaz F, Almeida-Porada G, Srour EF, Zanjani ED, Ascensão JL. Human natural killer cell development in a xenogeneic culture system. Br J Haematol. 2002 Sep; 118(3):885-92. PMID: 12181062.
      Citations:    
    105. McNiece IK, Almeida-Porada G, Shpall EJ, Zanjani E. Ex vivo expanded cord blood cells provide rapid engraftment in fetal sheep but lack long-term engrafting potential. Exp Hematol. 2002 Jun; 30(6):612-6. PMID: 12063029.
      Citations:    
    106. Porada CD, Tran ND, Almeida-Porada G, Glimp HA, Pixley JS, Zhao Y, Anderson WF, Zanjani ED. Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors. Hum Gene Ther. 2002 May 01; 13(7):867-79. PMID: 11975852.
      Citations:    
    107. Lewis ID, Almeida-Porada G, Du J, Lemischka IR, Moore KA, Zanjani ED, Verfaillie CM. Umbilical cord blood cells capable of engrafting in primary, secondary, and tertiary xenogeneic hosts are preserved after ex vivo culture in a noncontact system. Blood. 2001 Jun 01; 97(11):3441-9. PMID: 11369635.
      Citations:    
    108. Giesert C, Almeida-Porada G, Scheffold A, Kanz L, Zanjani ED, Bühring HJ. The monoclonal antibody W7C5 defines a novel surface antigen on hematopoietic stem cells. Ann N Y Acad Sci. 2001 Jun; 938:175-83. PMID: 11458505.
      Citations:    
    109. Tran ND, Porada CD, Almeida-Porada G, Glimp HA, Anderson WF, Zanjani ED. Induction of stable prenatal tolerance to beta-galactosidase by in utero gene transfer into preimmune sheep fetuses. Blood. 2001 Jun 01; 97(11):3417-23. PMID: 11369632.
      Citations:    
    110. Almeida-Porada G, Porada C, Zanjani ED. Adult stem cell plasticity and methods of detection. Rev Clin Exp Hematol. 2001 Mar; 5(1):26-41. PMID: 11486730.
      Citations:    
    111. Almeida-Porada G, Brown RL, MacKintosh FR, Zanjani ED. Evaluation of serum-free culture conditions able to support the ex vivo expansion and engraftment of human hematopoietic stem cells in the human-to-sheep xenograft model. J Hematother Stem Cell Res. 2000 Oct; 9(5):683-93. PMID: 11091492.
      Citations:    
    112. Verfaillie CM, Almeida-Porada G, Wissink S, Zanjani ED. Kinetics of engraftment of CD34(-) and CD34(+) cells from mobilized blood differs from that of CD34(-) and CD34(+) cells from bone marrow. Exp Hematol. 2000 Sep; 28(9):1071-9. PMID: 11008020.
      Citations:    
    113. Almeida-Porada G, Porada CD, Tran N, Zanjani ED. Cotransplantation of human stromal cell progenitors into preimmune fetal sheep results in early appearance of human donor cells in circulation and boosts cell levels in bone marrow at later time points after transplantation. Blood. 2000 Jun 01; 95(11):3620-7. PMID: 10828053.
      Citations:    
    114. Tran ND, Porada CD, Zhao Y, Almeida-Porada G, Anderson WF, Zanjani ED. In utero transfer and expression of exogenous genes in sheep. Exp Hematol. 2000 Jan; 28(1):17-30. PMID: 10658673.
      Citations:    
    115. Zanjani ED, Flake AW, Almeida-Porada G, Tran N, Papayannopoulou T. Homing of human cells in the fetal sheep model: modulation by antibodies activating or inhibiting very late activation antigen-4-dependent function. Blood. 1999 Oct 01; 94(7):2515-22. PMID: 10498625.
      Citations:    
    116. Almeida-Porada G, Flake AW, Glimp HA, Zanjani ED. Cotransplantation of stroma results in enhancement of engraftment and early expression of donor hematopoietic stem cells in utero. Exp Hematol. 1999 Oct; 27(10):1569-75. PMID: 10517499.
      Citations:    
    117. Ziegler BL, Valtieri M, Porada GA, De Maria R, Müller R, Masella B, Gabbianelli M, Casella I, Pelosi E, Bock T, Zanjani ED, Peschle C. KDR receptor: a key marker defining hematopoietic stem cells. Science. 1999 Sep 03; 285(5433):1553-8. PMID: 10477517.
      Citations:    
    118. Zanjani ED, Almeida-Porada G, Livingston AG, Porada CD, Ogawa M. Engraftment and multilineage expression of human bone marrow CD34- cells in vivo. Ann N Y Acad Sci. 1999 Apr 30; 872:220-31; discussion 231-2. PMID: 10372125.
      Citations:    
    119. Prudhomme JG, Almeida-Porada G, Ascensao JL, Sherman IW. Plasmodium-falciparum-infected erythrocytes adhere to immortalized human bone marrow endothelial cells. In Vitro Cell Dev Biol Anim. 1998 Jul-Aug; 34(7):524-6. PMID: 9719410.
      Citations:    
    120. Vaz F, Srour EF, Almeida-Porada G, Ascensao JL. Human thymic stroma supports human natural killer (NK) cell development from immature progenitors. Cell Immunol. 1998 Jun 15; 186(2):133-9. PMID: 9665755.
      Citations:    
    121. Shimizu Y, Ogawa M, Kobayashi M, Almeida-Porada G, Zanjani ED. Engraftment of cultured human hematopoietic cells in sheep. Blood. 1998 May 15; 91(10):3688-92. PMID: 9573005.
      Citations:    
    122. Zanjani ED, Almeida-Porada G, Livingston AG, Flake AW, Ogawa M. Human bone marrow CD34- cells engraft in vivo and undergo multilineage expression that includes giving rise to CD34+ cells. Exp Hematol. 1998 Apr; 26(4):353-60. PMID: 9546319.
      Citations:    
    123. Vaz F, Hoffman R, Almeida-Porada G, Ascensao JL. Definition of early progenitors and functional maturation of human natural killer cells: requirements for cytocidal activity. Pathobiology. 1998; 66(1):41-8. PMID: 9577966.
      Citations:    
    124. Yin AH, Miraglia S, Zanjani ED, Almeida-Porada G, Ogawa M, Leary AG, Olweus J, Kearney J, Buck DW. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood. 1997 Dec 15; 90(12):5002-12. PMID: 9389720.
      Citations:    
    125. Almeida-Porada G, Porada CD, Shanley JD, Ascensão JL. Altered production of GM-CSF and IL-8 in cytomegalovirus-infected, IL-1-primed umbilical cord endothelial cells. Exp Hematol. 1997 Nov; 25(12):1278-85. PMID: 9357972.
      Citations:    
    126. Zanjani ED, Almeida-Porada G, Ascensao JL, MacKintosh FR, Flake AW. Transplantation of hematopoietic stem cells in utero. Stem Cells. 1997; 15 Suppl 1:79-92; discussion 93. PMID: 9368328.
      Citations:    
    127. Flake AW, Roncarolo MG, Puck JM, Almeida-Porada G, Evans MI, Johnson MP, Abella EM, Harrison DD, Zanjani ED. Treatment of X-linked severe combined immunodeficiency by in utero transplantation of paternal bone marrow. N Engl J Med. 1996 Dec 12; 335(24):1806-10. PMID: 8943162.
      Citations:    
    128. Civin CI, Almeida-Porada G, Lee MJ, Olweus J, Terstappen LW, Zanjani ED. Sustained, retransplantable, multilineage engraftment of highly purified adult human bone marrow stem cells in vivo. Blood. 1996 Dec 01; 88(11):4102-9. PMID: 8943843.
      Citations:    
    129. Almeida-Porada G, Ascensão JL. Isolation, characterization, and biologic features of bone marrow endothelial cells. J Lab Clin Med. 1996 Oct; 128(4):399-407. PMID: 8833889.
      Citations:    
    130. Almeida-Porada G, Ascensao JL, Zanjani ED. The role of sheep stroma in human haemopoiesis in the human/sheep chimaeras. Br J Haematol. 1996 Jun; 93(4):795-802. PMID: 8703805.
      Citations:    
    131. Zanjani ED, Almeida-Porada G, Flake AW. The human/sheep xenograft model: a large animal model of human hematopoiesis. Int J Hematol. 1996 Apr; 63(3):179-92. PMID: 8936332.
      Citations:    
    132. Almeida-Porada GD, Ascensao JL. Cytomegalovirus as a cause of pancytopenia. Leuk Lymphoma. 1996 Apr; 21(3-4):217-23. PMID: 8726402.
      Citations:    
    133. Almeida-Porada GD, Hoffman R, Manalo P, Gianni AM, Zanjani ED. Detection of human cells in human/sheep chimeric lambs with in vitro human stroma-forming potential. Exp Hematol. 1996 Feb; 24(3):482-7. PMID: 8599979.
      Citations:    
    134. Zanjani ED, Almeida-Porada G, Flake AW. Retention and multilineage expression of human hematopoietic stem cells in human-sheep chimeras. Stem Cells. 1995 Mar; 13(2):101-11. PMID: 7787777.
      Citations:    
    135. Bilgrami S, Almeida GD, Quinn JJ, Tuck D, Bergstrom S, Dainiak N, Poliquin C, Ascensao JL. Pancytopenia in allogeneic marrow transplant recipients: role of cytomegalovirus. Br J Haematol. 1994 Jun; 87(2):357-62. PMID: 7947279.
      Citations:    
    136. Almeida GD, Porada CD, St Jeor S, Ascensao JL. Human cytomegalovirus alters interleukin-6 production by endothelial cells. Blood. 1994 Jan 15; 83(2):370-6. PMID: 8286737.
      Citations:    
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