Polymerase Chain Reaction
"Polymerase Chain Reaction" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus,
MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure,
which enables searching at various levels of specificity.
In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
|Polymerase Chain Reaction
- Polymerase Chain Reaction
- Polymerase Chain Reactions
- Reaction, Polymerase Chain
- Reactions, Polymerase Chain
- Inverse PCR
- PCR, Inverse
- Inverse Polymerase Chain Reaction
- Anchored PCR
- PCR, Anchored
- Anchored Polymerase Chain Reaction
Below are MeSH descriptors whose meaning is more general than "Polymerase Chain Reaction".
Below are MeSH descriptors whose meaning is more specific than "Polymerase Chain Reaction".
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Below are the most recent publications written about "Polymerase Chain Reaction" by people in Profiles.