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Clinical and Translational Science Institute

Last Name

Christopher Porada, Ph.D.

TitleAssociate Professor
InstitutionWake Forest School of Medicine
DepartmentWF Institute for Regen Medicine
Medical Center Blvd
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    Other Positions
    TitleAssociate Professor
    InstitutionWake Forest School of Medicine
    DepartmentRadiologic Sciences-Medical Engineering

    Collapse Research 
    Collapse research activities and funding
    R01HD043038     (PORADA, CHRISTOPHER D)Mar 1, 2002 - Feb 28, 2008
    In Utero Gene Therapy
    Role: Principal Investigator

    R01HL130856     (ALMEIDA-PORADA, GRACA DUARTE)Sep 1, 2016 - May 31, 2020
    Postnatal Cell-Based Therapies for Hemophilia A
    Role: Co-Principal Investigator

    R01HL135853     (ALMEIDA-PORADA, GRACA DUARTE)Jul 14, 2017 - Jun 30, 2021
    Prenatal Cell and Gene Therapy for Hemophilia A
    Role: Co-Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Rodman C, Almeida-Porada G, George SK, Moon J, Soker S, Pardee T, Beaty M, Guida P, Sajuthi SP, Langefeld CD, Walker SJ, Wilson PF, Porada CD. In vitro and in vivo assessment of direct effects of simulated solar and galactic cosmic radiation on human hematopoietic stem/progenitor cells. Leukemia. 2017 Jun; 31(6):1398-1407. PMID: 27881872.
      View in: PubMed
    2. Mokhtari S, Colletti EJ, Atala A, Zanjani ED, Porada CD, Almeida-Porada G. Boosting Hematopoietic Engraftment after in Utero Transplantation through Vascular Niche Manipulation. Stem Cell Reports. 2016 06 14; 6(6):957-69. PMID: 27304918; PMCID: PMC4912311.
    3. Almeida-Porada G, Atala A, Porada CD. In utero stem cell transplantation and gene therapy: rationale, history, and recent advances toward clinical application. Mol Ther Methods Clin Dev. 2016; 5:16020. PMID: 27069953; PMCID: PMC4813605.
    4. Muralidharan S, Sasi SP, Zuriaga MA, Hirschi KK, Porada CD, Coleman MA, Walsh KX, Yan X, Goukassian DA. Corrigendum: Ionizing Particle Radiation as a Modulator of Endogenous Bone Marrow Cell Reprogramming: Implications for Hematological Cancers. Front Oncol. 2015; 5:255. PMID: 26636037; PMCID: PMC4658418.
    5. Muralidharan S, Sasi SP, Zuriaga MA, Hirschi KK, Porada CD, Coleman MA, Walsh KX, Yan X, Goukassian DA. Ionizing Particle Radiation as a Modulator of Endogenous Bone Marrow Cell Reprogramming: Implications for Hematological Cancers. Front Oncol. 2015; 5:231. PMID: 26528440; PMCID: PMC4604322.
    6. Porada CD, Atala AJ, Almeida-Porada G. The hematopoietic system in the context of regenerative medicine. Methods. 2016 Apr 15; 99:44-61. PMID: 26319943; PMCID: PMC4769971 [Available on 04/15/17].
    7. Ross CL, Siriwardane M, Almeida-Porada G, Porada CD, Brink P, Christ GJ, Harrison BS. The effect of low-frequency electromagnetic field on human bone marrow stem/progenitor cell differentiation. Stem Cell Res. 2015 Jul; 15(1):96-108. PMID: 26042793; PMCID: PMC4516580.
    8. Shaw SW, Blundell MP, Pipino C, Shangaris P, Maghsoudlou P, Ramachandra DL, Georgiades F, Boyd M, Thrasher AJ, Porada CD, Almeida-Porada G, Cheng PJ, David AL, de Coppi P. Sheep CD34+ amniotic fluid cells have hematopoietic potential and engraft after autologous in utero transplantation. Stem Cells. 2015 Jan; 33(1):122-32. PMID: 25186828.
      View in: PubMed
    9. Porada CD, Rodman C, Ignacio G, Atala A, Almeida-Porada G. Hemophilia A: an ideal disease to correct in utero. Front Pharmacol. 2014; 5:276. PMID: 25566073; PMCID: PMC4263089.
    10. Boura JS, Vance M, Yin W, Madeira C, Lobato da Silva C, Porada CD, Almeida-Porada G. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev. 2014 Sep; 2014(1). PMID: 25279386.
      View in: PubMed
    11. Jeanblanc C, Goodrich AD, Colletti E, Mokhtari S, Porada CD, Zanjani ED, Almeida-Porada G. Temporal definition of haematopoietic stem cell niches in a large animal model of in utero stem cell transplantation. Br J Haematol. 2014 Jul; 166(2):268-78. PMID: 24673111; PMCID: PMC4079736.
    12. Soland MA, Keyes LR, Bayne R, Moon J, Porada CD, St Jeor S, Almeida-Porada G. Perivascular stromal cells as a potential reservoir of human cytomegalovirus. Am J Transplant. 2014 Apr; 14(4):820-30. PMID: 24592822; PMCID: PMC4046334.
    13. Porada CD, Stem C, Almeida-Porada G. Gene therapy: the promise of a permanent cure. N C Med J. 2013 Nov-Dec; 74(6):526-9. PMID: 24316783.
      View in: PubMed
    14. Tellez J, Van Vliet K, Tseng YS, Finn JD, Tschernia N, Almeida-Porada G, Arruda VR, Agbandje-McKenna M, Porada CD. Characterization of naturally-occurring humoral immunity to AAV in sheep. PLoS One. 2013; 8(9):e75142. PMID: 24086458; PMCID: PMC3782463.
    15. Almeida-Porada G, Soland M, Boura J, Porada CD. Regenerative medicine: prospects for the treatment of inflammatory bowel disease. Regen Med. 2013 Sep; 8(5):631-44. PMID: 23998755.
      View in: PubMed
    16. Sanada C, Kuo CJ, Colletti EJ, Soland M, Mokhtari S, Knovich MA, Owen J, Zanjani ED, Porada CD, Almeida-Porada G. Mesenchymal stem cells contribute to endogenous FVIII:c production. J Cell Physiol. 2013 May; 228(5):1010-6. PMID: 23042590; PMCID: PMC3954540.
    17. Soland MA, Bego M, Colletti E, Zanjani ED, St Jeor S, Porada CD, Almeida-Porada G. Mesenchymal stem cells engineered to inhibit complement-mediated damage. PLoS One. 2013; 8(3):e60461. PMID: 23555976; PMCID: PMC3608620.
    18. Colletti E, El Shabrawy D, Soland M, Yamagami T, Mokhtari S, Osborne C, Schlauch K, Zanjani ED, Porada CD, Almeida-Porada G. EphB2 isolates a human marrow stromal cell subpopulation with enhanced ability to contribute to the resident intestinal cellular pool. FASEB J. 2013 Jun; 27(6):2111-21. PMID: 23413357; PMCID: PMC3659358.
    19. Zakas PM, Gangadharan B, Almeida-Porada G, Porada CD, Spencer HT, Doering CB. Development and characterization of recombinant ovine coagulation factor VIII. PLoS One. 2012; 7(11):e49481. PMID: 23152911; PMCID: PMC3494657.
    20. Wood JA, Colletti E, Mead LE, Ingram D, Porada CD, Zanjani ED, Yoder MC, Almeida-Porada G. Distinct contribution of human cord blood-derived endothelial colony forming cells to liver and gut in a fetal sheep model. Hepatology. 2012 Sep; 56(3):1086-96. PMID: 22488442; PMCID: PMC3396735.
    21. Soland MA, Bego MG, Colletti E, Porada CD, Zanjani ED, St Jeor S, Almeida-Porada G. Modulation of human mesenchymal stem cell immunogenicity through forced expression of human cytomegalovirus us proteins. PLoS One. 2012; 7(5):e36163. PMID: 22666319; PMCID: PMC3364258.
    22. Porada CD, Almeida-Porada G. Treatment of Hemophilia A in Utero and Postnatally using Sheep as a Model for Cell and Gene Delivery. J Genet Syndr Gene Ther. 2012 May 25; S1. PMID: 23264887.
      View in: PubMed
    23. Ozturk F, Park PJ, Tellez J, Colletti E, Eiden MV, Almeida-Porada G, Porada CD. Expression levels of the PiT-2 receptor explain, in part, the gestational age-dependent alterations in transduction efficiency after in utero retroviral-mediated gene transfer. J Gene Med. 2012 Mar; 14(3):169-81. PMID: 22262359; PMCID: PMC3934108.
    24. Porada CD, Sanada C, Kuo CJ, Colletti E, Mandeville W, Hasenau J, Zanjani ED, Moot R, Doering C, Spencer HT, Almeida-Porada G. Phenotypic correction of hemophilia A in sheep by postnatal intraperitoneal transplantation of FVIII-expressing MSC. Exp Hematol. 2011 Dec; 39(12):1124-1135.e4. PMID: 21906573; PMCID: PMC3220800.
    25. Porada CD, Almeida-Porada G. Mesenchymal stem cells as therapeutics and vehicles for gene and drug delivery. Adv Drug Deliv Rev. 2010 Sep 30; 62(12):1156-66. PMID: 20828588; PMCID: PMC2991625.
    26. Almeida-Porada G, Zanjani ED, Porada CD. Bone marrow stem cells and liver regeneration. Exp Hematol. 2010 Jul; 38(7):574-80. PMID: 20417684; PMCID: PMC2882990.
    27. Goodrich AD, Ersek A, Varain NM, Groza D, Cenariu M, Thain DS, Almeida-Porada G, Porada CD, Zanjani ED. In vivo generation of beta-cell-like cells from CD34(+) cells differentiated from human embryonic stem cells. Exp Hematol. 2010 Jun; 38(6):516-525.e4. PMID: 20227460; PMCID: PMC2880573.
    28. Ersek A, Pixley JS, Goodrich AD, Porada CD, Almeida-Porada G, Thain DS, Zanjani ED. Persistent circulating human insulin in sheep transplanted in utero with human mesenchymal stem cells. Exp Hematol. 2010 Apr; 38(4):311-20. PMID: 20170708; PMCID: PMC2854135.
    29. Porada CD, Sanada C, Long CR, Wood JA, Desai J, Frederick N, Millsap L, Bormann C, Menges SL, Hanna C, Flores-Foxworth G, Shin T, Westhusin ME, Liu W, Glimp H, Zanjani ED, Lozier JN, Pliska V, Stranzinger G, Joerg H, Kraemer DC, Almeida-Porada G. Clinical and molecular characterization of a re-established line of sheep exhibiting hemophilia A. J Thromb Haemost. 2010 Feb; 8(2):276-85. PMID: 19943872; PMCID: PMC2826196.
    30. da Silva CL, Gonçalves R, Porada CD, Ascensão JL, Zanjani ED, Cabral JM, Almeida-Porada G. Differences amid bone marrow and cord blood hematopoietic stem/progenitor cell division kinetics. J Cell Physiol. 2009 Jul; 220(1):102-11. PMID: 19277981; PMCID: PMC4008884.
    31. Park PJ, Colletti E, Ozturk F, Wood JA, Tellez J, Almeida-Porada G, Porada C. Factors determining the risk of inadvertent retroviral transduction of male germ cells after in utero gene transfer in sheep. Hum Gene Ther. 2009 Mar; 20(3):201-15. PMID: 19301473; PMCID: PMC2829302.
    32. Yamagami T, Porada CD, Pardini RS, Zanjani ED, Almeida-Porada G. Docosahexaenoic acid induces dose dependent cell death in an early undifferentiated subtype of acute myeloid leukemia cell line. Cancer Biol Ther. 2009 Feb; 8(4):331-7. PMID: 19197149; PMCID: PMC3954154.
    33. Porada CD, Harrison-Findik DD, Sanada C, Valiente V, Thain D, Simmons PJ, Almeida-Porada G, Zanjani ED. Development and characterization of a novel CD34 monoclonal antibody that identifies sheep hematopoietic stem/progenitor cells. Exp Hematol. 2008 Dec; 36(12):1739-49. PMID: 19007686; PMCID: PMC2596892.
    34. Colletti EJ, Airey JA, Liu W, Simmons PJ, Zanjani ED, Porada CD, Almeida-Porada G. Generation of tissue-specific cells from MSC does not require fusion or donor-to-host mitochondrial/membrane transfer. Stem Cell Res. 2009 Mar; 2(2):125-38. PMID: 19383418; PMCID: PMC3969729.
    35. Colletti E, Lindstedt S, Park PJ, Almeida-Porada G, Porada CD. Early fetal gene delivery utilizes both central and peripheral mechanisms of tolerance induction. Exp Hematol. 2008 Jul; 36(7):816-22. PMID: 18400364; PMCID: PMC2486147.
    36. Frias AM, Porada CD, Crapnell KB, Cabral JM, Zanjani ED, Almeida-Porada G. Generation of functional natural killer and dendritic cells in a human stromal-based serum-free culture system designed for cord blood expansion. Exp Hematol. 2008 Jan; 36(1):61-8. PMID: 18155552; PMCID: PMC2214851.
    37. Chamberlain J, Yamagami T, Colletti E, Theise ND, Desai J, Frias A, Pixley J, Zanjani ED, Porada CD, Almeida-Porada G. Efficient generation of human hepatocytes by the intrahepatic delivery of clonal human mesenchymal stem cells in fetal sheep. Hepatology. 2007 Dec; 46(6):1935-45. PMID: 17705296.
      View in: PubMed
    38. Almeida-Porada G, Porada C, Gupta N, Torabi A, Thain D, Zanjani ED. The human-sheep chimeras as a model for human stem cell mobilization and evaluation of hematopoietic grafts' potential. Exp Hematol. 2007 Oct; 35(10):1594-600. PMID: 17889724; PMCID: PMC2048750.
    39. Porada CD, Zanjani ED, Almeida-Porad G. Adult mesenchymal stem cells: a pluripotent population with multiple applications. Curr Stem Cell Res Ther. 2006 Sep; 1(3):365-9. PMID: 18220880.
      View in: PubMed
    40. Porada CD, Park PJ, Tellez J, Ozturk F, Glimp HA, Almeida-Porada G, Zanjani ED. Male germ-line cells are at risk following direct-injection retroviral-mediated gene transfer in utero. Mol Ther. 2005 Oct; 12(4):754-62. PMID: 16084775.
      View in: PubMed
    41. Lucas ML, Seidel NE, Porada CD, Quigley JG, Anderson SM, Malech HL, Abkowitz JL, Zanjani ED, Bodine DM. Improved transduction of human sheep repopulating cells by retrovirus vectors pseudotyped with feline leukemia virus type C or RD114 envelopes. Blood. 2005 Jul 01; 106(1):51-8. PMID: 15774617; PMCID: PMC1895126.
    42. Porada CD, Park PJ, Almeida-Porada G, Liu W, Ozturk F, Glimp HA, Zanjani ED. Gestational age of recipient determines pattern and level of transgene expression following in utero retroviral gene transfer. Mol Ther. 2005 Feb; 11(2):284-93. PMID: 15668140.
      View in: PubMed
    43. Almeida-Porada G, Porada CD, Chamberlain J, Torabi A, Zanjani ED. Formation of human hepatocytes by human hematopoietic stem cells in sheep. Blood. 2004 Oct 15; 104(8):2582-90. PMID: 15231580.
      View in: PubMed
    44. Porada GA, Porada C, Zanjani ED. The fetal sheep: a unique model system for assessing the full differentiative potential of human stem cells. Yonsei Med J. 2004 Jun 30; 45 Suppl:7-14. PMID: 15250043.
      View in: PubMed
    45. Airey JA, Almeida-Porada G, Colletti EJ, Porada CD, Chamberlain J, Movsesian M, Sutko JL, Zanjani ED. Human mesenchymal stem cells form Purkinje fibers in fetal sheep heart. Circulation. 2004 Mar 23; 109(11):1401-7. PMID: 15023887.
      View in: PubMed
    46. Almeida-Porada G, Porada C, Zanjani ED. Plasticity of human stem cells in the fetal sheep model of human stem cell transplantation. Int J Hematol. 2004 Jan; 79(1):1-6. PMID: 14979471.
      View in: PubMed
    47. Porada CD, Park P, Almeida-Porada G, Zanjani ED. The sheep model of in utero gene therapy. Fetal Diagn Ther. 2004 Jan-Feb; 19(1):23-30. PMID: 14646413.
      View in: PubMed
    48. Almeida-Porada G, El Shabrawy D, Porada C, Zanjani ED. Differentiative potential of human metanephric mesenchymal cells. Exp Hematol. 2002 Dec; 30(12):1454-62. PMID: 12482508.
      View in: PubMed
    49. Tsai EJ, Malech HL, Kirby MR, Hsu AP, Seidel NE, Porada CD, Zanjani ED, Bodine DM, Puck JM. Retroviral transduction of IL2RG into CD34(+) cells from X-linked severe combined immunodeficiency patients permits human T- and B-cell development in sheep chimeras. Blood. 2002 Jul 01; 100(1):72-9. PMID: 12070011.
      View in: PubMed
    50. Porada CD, Tran ND, Almeida-Porada G, Glimp HA, Pixley JS, Zhao Y, Anderson WF, Zanjani ED. Transduction of long-term-engrafting human hematopoietic stem cells by retroviral vectors. Hum Gene Ther. 2002 May 01; 13(7):867-79. PMID: 11975852.
      View in: PubMed
    51. Tran ND, Porada CD, Almeida-Porada G, Glimp HA, Anderson WF, Zanjani ED. Induction of stable prenatal tolerance to beta-galactosidase by in utero gene transfer into preimmune sheep fetuses. Blood. 2001 Jun 01; 97(11):3417-23. PMID: 11369632.
      View in: PubMed
    52. Porada CD, Tran ND, Zhao Y, Anderson WF, Zanjani ED. Neonatal gene therapy. transfer and expression of exogenous genes in neonatal sheep following direct injection of retroviral vectors into the bone marrow space. Exp Hematol. 2000 Jun; 28(6):642-50. PMID: 10880750.
      View in: PubMed
    53. Almeida-Porada G, Porada CD, Tran N, Zanjani ED. Cotransplantation of human stromal cell progenitors into preimmune fetal sheep results in early appearance of human donor cells in circulation and boosts cell levels in bone marrow at later time points after transplantation. Blood. 2000 Jun 01; 95(11):3620-7. PMID: 10828053.
      View in: PubMed
    54. Tran ND, Porada CD, Zhao Y, Almeida-Porada G, Anderson WF, Zanjani ED. In utero transfer and expression of exogenous genes in sheep. Exp Hematol. 2000 Jan; 28(1):17-30. PMID: 10658673.
      View in: PubMed
    55. Zanjani ED, Almeida-Porada G, Livingston AG, Porada CD, Ogawa M. Engraftment and multilineage expression of human bone marrow CD34- cells in vivo. Ann N Y Acad Sci. 1999 Apr 30; 872:220-31; discussion 231-2. PMID: 10372125.
      View in: PubMed
    56. Porada CD, Tran N, Eglitis M, Moen RC, Troutman L, Flake AW, Zhao Y, Anderson WF, Zanjani ED. In utero gene therapy: transfer and long-term expression of the bacterial neo(r) gene in sheep after direct injection of retroviral vectors into preimmune fetuses. Hum Gene Ther. 1998 Jul 20; 9(11):1571-85. PMID: 9694156.
      View in: PubMed
    57. Almeida-Porada G, Porada CD, Shanley JD, Ascensão JL. Altered production of GM-CSF and IL-8 in cytomegalovirus-infected, IL-1-primed umbilical cord endothelial cells. Exp Hematol. 1997 Nov; 25(12):1278-85. PMID: 9357972.
      View in: PubMed
    58. Almeida GD, Porada CD, St Jeor S, Ascensao JL. Human cytomegalovirus alters interleukin-6 production by endothelial cells. Blood. 1994 Jan 15; 83(2):370-6. PMID: 8286737.
      View in: PubMed
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