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Thomas Hollis PhD

TitleProfessor
InstitutionWake Forest School of Medicine
DepartmentBiochemistry
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    Collapse Research 
    Collapse research activities and funding
    T32GM095440     (HOLLIS, THOMAS J)Jul 1, 2011 - Jun 30, 2021
    NIH
    Structural and Computational Biophysics Training Program
    Role: Principal Investigator
    R01GM108827     (HOLLIS, THOMAS J)Sep 15, 2014 - Aug 31, 2019
    NIH
    Structure and Regulation of the TREX1 Exonuclease
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Mauney CH, Perrino FW, Hollis T. Identification of Inhibitors of the dNTP Triphosphohydrolase SAMHD1 Using a Novel and Direct High-Throughput Assay. Biochemistry. 2018 11 27; 57(47):6624-6636. PMID: 30380297.
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    2. Guragain M, Jennings-Gee J, Cattelan N, Finger M, Conover MS, Hollis T, Deora R. The Transcriptional Regulator BpsR Controls the Growth of Bordetella bronchiseptica by Repressing Genes Involved in Nicotinic Acid Degradation. J Bacteriol. 2018 06 15; 200(12). PMID: 29581411.
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    3. Mauney CH, Hollis T. SAMHD1: Recurring roles in cell cycle, viral restriction, cancer, and innate immunity. Autoimmunity. 2018 05; 51(3):96-110. PMID: 29583030.
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    4. Rogers LC, Davis RR, Said N, Hollis T, Daniel LW. Blocking LPA-dependent signaling increases ovarian cancer cell death in response to chemotherapy. Redox Biol. 2018 05; 15:380-386. PMID: 29331665.
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    5. Wang F, Zahid OK, Swain BE, Parsonage D, Hollis T, Harvey S, Perrino FW, Kohli RM, Taylor EW, Hall AR. Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process. . 2017 11 08; 17(11):7110-7116. PMID: 28967259.
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    6. Mauney CH, Rogers LC, Harris RS, Daniel LW, Devarie-Baez NO, Wu H, Furdui CM, Poole LB, Perrino FW, Hollis T. The SAMHD1 dNTP Triphosphohydrolase Is Controlled by a Redox Switch. Antioxid Redox Signal. 2017 Dec 01; 27(16):1317-1331. PMID: 28398823.
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    7. Grieves JL, Fye JM, Harvey S, Grayson JM, Hollis T, Perrino FW. Exonuclease TREX1 degrades double-stranded DNA to prevent spontaneous lupus-like inflammatory disease. Proc Natl Acad Sci U S A. 2015 Apr 21; 112(16):5117-22. PMID: 25848017.
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    8. Davis RR, Shaban NM, Perrino FW, Hollis T. Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding. . 2015; 14(4):668-73. PMID: 25664393.
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    9. Huhn AJ, Parsonage D, Horita DA, Torti FM, Torti SV, Hollis T. The high-molecular-weight kininogen domain 5 is an intrinsically unstructured protein and its interaction with ferritin is metal mediated. Protein Sci. 2014 Aug; 23(8):1013-22. PMID: 24810540.
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    10. Limoli DH, Rockel AB, Host KM, Jha A, Kopp BT, Hollis T, Wozniak DJ. Cationic antimicrobial peptides promote microbial mutagenesis and pathoadaptation in chronic infections. PLoS Pathog. 2014 Apr; 10(4):e1004083. PMID: 24763694.
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    11. Fye JM, Coffin SR, Orebaugh CD, Hollis T, Perrino FW. The Arg-62 residues of the TREX1 exonuclease act across the dimer interface contributing to catalysis in the opposing protomers. J Biol Chem. 2014 Apr 18; 289(16):11556-11565. PMID: 24616097.
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    12. Orebaugh CD, Fye JM, Harvey S, Hollis T, Wilkinson JC, Perrino FW. The TREX1 C-terminal region controls cellular localization through ubiquitination. J Biol Chem. 2013 Oct 04; 288(40):28881-92. PMID: 23979357.
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    13. Pryor EE, Wozniak DJ, Hollis T. Crystallization of Pseudomonas aeruginosa AmrZ protein: development of a comprehensive method for obtaining and optimization of protein-DNA crystals. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Aug 01; 68(Pt 8):985-93. PMID: 22869139.
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    14. Pryor EE, Waligora EA, Xu B, Dellos-Nolan S, Wozniak DJ, Hollis T. The transcription factor AmrZ utilizes multiple DNA binding modes to recognize activator and repressor sequences of Pseudomonas aeruginosa virulence genes. PLoS Pathog. 2012; 8(4):e1002648. PMID: 22511872.
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    15. Bailey SL, Harvey S, Perrino FW, Hollis T. Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease. DNA Repair (Amst). 2012 Jan 02; 11(1):65-73. PMID: 22071149.
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    16. Powell RD, Holland PJ, Hollis T, Perrino FW. Aicardi-Goutieres syndrome gene and HIV-1 restriction factor SAMHD1 is a dGTP-regulated deoxynucleotide triphosphohydrolase. J Biol Chem. 2011 Dec 23; 286(51):43596-43600. PMID: 22069334.
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    17. Orebaugh CD, Fye JM, Harvey S, Hollis T, Perrino FW. The TREX1 exonuclease R114H mutation in Aicardi-Goutières syndrome and lupus reveals dimeric structure requirements for DNA degradation activity. J Biol Chem. 2011 Nov 18; 286(46):40246-54. PMID: 21937424.
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    18. Fye JM, Orebaugh CD, Coffin SR, Hollis T, Perrino FW. Dominant mutation of the TREX1 exonuclease gene in lupus and Aicardi-Goutieres syndrome. J Biol Chem. 2011 Sep 16; 286(37):32373-82. PMID: 21808053.
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    19. Coffin SR, Hollis T, Perrino FW. Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome. J Biol Chem. 2011 May 13; 286(19):16984-91. PMID: 21454563.
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    20. Waligora EA, Ramsey DM, Pryor EE, Lu H, Hollis T, Sloan GP, Deora R, Wozniak DJ. AmrZ beta-sheet residues are essential for DNA binding and transcriptional control of Pseudomonas aeruginosa virulence genes. J Bacteriol. 2010 Oct; 192(20):5390-401. PMID: 20709902.
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    21. Holland PJ, Hollis T. Structural and mutational analysis of Escherichia coli AlkB provides insight into substrate specificity and DNA damage searching. PLoS One. 2010 Jan 13; 5(1):e8680. PMID: 20084272.
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    22. Shaban NM, Harvey S, Perrino FW, Hollis T. The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction. J Biol Chem. 2010 Feb 05; 285(6):3617-3624. PMID: 19923215.
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    23. de Silva U, Perrino FW, Hollis T. DNA binding induces active site conformational change in the human TREX2 3'-exonuclease. Nucleic Acids Res. 2009 Apr; 37(7):2411-7. PMID: 19321497.
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    24. Perrino FW, Harvey S, Shaban NM, Hollis T. RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases. J Mol Med (Berl). 2009 Jan; 87(1):25-30. PMID: 19034401.
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    25. Vasilyeva A, Clodfelter JE, Rector B, Hollis T, Scarpinato KD, Salsbury FR. Small molecule induction of MSH2-dependent cell death suggests a vital role of mismatch repair proteins in cell death. DNA Repair (Amst). 2009 Jan 01; 8(1):103-13. PMID: 18955167.
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    26. Pence MG, Blans P, Zink CN, Hollis T, Fishbein JC, Perrino FW. Lesion bypass of N2-ethylguanine by human DNA polymerase iota. J Biol Chem. 2009 Jan 16; 284(3):1732-40. PMID: 18984581.
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    27. Lehtinen DA, Harvey S, Mulcahy MJ, Hollis T, Perrino FW. The TREX1 double-stranded DNA degradation activity is defective in dominant mutations associated with autoimmune disease. J Biol Chem. 2008 Nov 14; 283(46):31649-56. PMID: 18805785.
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    28. Perrino FW, de Silva U, Harvey S, Pryor EE, Cole DW, Hollis T. Cooperative DNA binding and communication across the dimer interface in the TREX2 3' --> 5'-exonuclease. J Biol Chem. 2008 Aug 01; 283(31):21441-52. PMID: 18534978.
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    29. Lee-Kirsch MA, Gong M, Chowdhury D, Senenko L, Engel K, Lee YA, de Silva U, Bailey SL, Witte T, Vyse TJ, Kere J, Pfeiffer C, Harvey S, Wong A, Koskenmies S, Hummel O, Rohde K, Schmidt RE, Dominiczak AF, Gahr M, Hollis T, Perrino FW, Lieberman J, Hübner N. Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 are associated with systemic lupus erythematosus. Nat Genet. 2007 Sep; 39(9):1065-7. PMID: 17660818.
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    30. Lee-Kirsch MA, Chowdhury D, Harvey S, Gong M, Senenko L, Engel K, Pfeiffer C, Hollis T, Gahr M, Perrino FW, Lieberman J, Hubner N. A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus. J Mol Med (Berl). 2007 May; 85(5):531-7. PMID: 17440703.
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    31. Metz AH, Hollis T, Eichman BF. DNA damage recognition and repair by 3-methyladenine DNA glycosylase I (TAG). EMBO J. 2007 May 02; 26(9):2411-20. PMID: 17410210.
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    32. Rice G, Newman WG, Dean J, Patrick T, Parmar R, Flintoff K, Robins P, Harvey S, Hollis T, O'Hara A, Herrick AL, Bowden AP, Perrino FW, Lindahl T, Barnes DE, Crow YJ. Heterozygous mutations in TREX1 cause familial chilblain lupus and dominant Aicardi-Goutieres syndrome. Am J Hum Genet. 2007 Apr; 80(4):811-5. PMID: 17357087.
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    33. de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007 Apr 06; 282(14):10537-43. PMID: 17293595.
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    34. Hollis T. Crystallization of protein-DNA complexes. Methods Mol Biol. 2007; 363:225-37. PMID: 17272844.
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    35. Salsbury FR, Clodfelter JE, Gentry MB, Hollis T, Scarpinato KD. The molecular mechanism of DNA damage recognition by MutS homologs and its consequences for cell death response. Nucleic Acids Res. 2006; 34(8):2173-85. PMID: 16648361.
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    36. Perrino FW, Harvey S, McMillin S, Hollis T. The human TREX2 3' -> 5'-exonuclease structure suggests a mechanism for efficient nonprocessive DNA catalysis. J Biol Chem. 2005 Apr 15; 280(15):15212-8. PMID: 15661738.
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    37. Perrino FW, Krol A, Harvey S, Zheng SL, Horita DA, Hollis T, Meyers DA, Isaacs WB, Xu J. Sequence variants in the 3'-->5' deoxyribonuclease TREX2: identification in a genetic screen and effects on catalysis by the recombinant proteins. Adv Enzyme Regul. 2004; 44:37-49. PMID: 15581481.
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    38. Rezende LF, Hollis T, Ellenberger T, Richardson CC. Essential amino acid residues in the single-stranded DNA-binding protein of bacteriophage T7. Identification of the dimer interface. J Biol Chem. 2002 Dec 27; 277(52):50643-53. PMID: 12379653.
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