Header Logo

Fred W.Perrino PhD

TitleProfessor
InstitutionWake Forest School of Medicine
DepartmentBiochemistry
Address
vCardDownload vCard

    Collapse Research 
    Collapse research activities and funding
    R01GM110734     (PERRINO, FRED W)May 1, 2014 - Apr 30, 2019
    NIH
    Cellular Nucleotide Metabolism in HIV Restriction
    Role: Principal Investigator
    R01AI116725     (PERRINO, FRED W)Jan 1, 2016 - Dec 31, 2020
    NIH
    The TREX1 Pathway in Autoimmune Disease
    Role: Principal Investigator

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    Newest   |   Oldest   |   Most Cited   |   Most Discussed   |   Timeline   |   Field Summary   |   Plain Text
    PMC Citations indicate the number of times the publication was cited by articles in PubMed Central, and the Altmetric score represents citations in news articles and social media. (Note that publications are often cited in additional ways that are not shown here.) Fields are based on how the National Library of Medicine (NLM) classifies the publication's journal and might not represent the specific topic of the publication. Translation tags are based on the publication type and the MeSH terms NLM assigns to the publication. Some publications (especially newer ones and publications not in PubMed) might not yet be assigned Field or Translation tags.) Click a Field or Translation tag to filter the publications.
    1. Rego SL, Harvey S, Simpson SR, Hemphill WO, McIver ZA, Grayson JM, Perrino FW. TREX1 D18N mice fail to process erythroblast DNA resulting in inflammation and dysfunctional erythropoiesis. Autoimmunity. 2018 Nov; 51(7):333-344. PMID: 30422000.
      Citations:    
    2. Mauney CH, Perrino FW, Hollis T. Identification of Inhibitors of the dNTP Triphosphohydrolase SAMHD1 Using a Novel and Direct High-Throughput Assay. Biochemistry. 2018 11 27; 57(47):6624-6636. PMID: 30380297.
      Citations:    
    3. Wang F, Zahid OK, Swain BE, Parsonage D, Hollis T, Harvey S, Perrino FW, Kohli RM, Taylor EW, Hall AR. Solid-State Nanopore Analysis of Diverse DNA Base Modifications Using a Modular Enzymatic Labeling Process. . 2017 11 08; 17(11):7110-7116. PMID: 28967259.
      Citations:    
    4. Mauney CH, Rogers LC, Harris RS, Daniel LW, Devarie-Baez NO, Wu H, Furdui CM, Poole LB, Perrino FW, Hollis T. The SAMHD1 dNTP Triphosphohydrolase Is Controlled by a Redox Switch. Antioxid Redox Signal. 2017 Dec 01; 27(16):1317-1331. PMID: 28398823.
      Citations:    
    5. Sakai T, Miyazaki T, Shin DM, Kim YS, Qi CF, Fariss R, Munasinghe J, Wang H, Kovalchuk AL, Kothari PH, Fermaintt CS, Atkinson JP, Perrino FW, Yan N, Morse HC. DNase-active TREX1 frame-shift mutants induce serologic autoimmunity in mice. J Autoimmun. 2017 Jul; 81:13-23. PMID: 28325644.
      Citations:    
    6. Manils J, Casas E, Viña-Vilaseca A, López-Cano M, Díez-Villanueva A, Gómez D, Marruecos L, Ferran M, Benito C, Perrino FW, Vavouri T, de Anta JM, Ciruela F, Soler C. The Exonuclease Trex2 Shapes Psoriatic Phenotype. J Invest Dermatol. 2016 12; 136(12):2345-2355. PMID: 27365293.
      Citations:    
    7. Manils J, Gómez D, Salla-Martret M, Fischer H, Fye JM, Marzo E, Marruecos L, Serrano I, Salgado R, Rodrigo JP, Garcia-Pedrero JM, Serafin AM, Cañas X, Benito C, Toll A, Forcales SV, Perrino FW, Eckhart L, Soler C. Multifaceted role of TREX2 in the skin defense against UV-induced skin carcinogenesis. Oncotarget. 2015 Sep 08; 6(26):22375-96. PMID: 26090614.
      Citations:    
    8. Harris RS, Perrino FW, Shaban NM. The multidimensional nature of antiviral innate immunity. . 2015 Apr 08; 17(4):423-5. PMID: 25856751.
      Citations:    
    9. Grieves JL, Fye JM, Harvey S, Grayson JM, Hollis T, Perrino FW. Exonuclease TREX1 degrades double-stranded DNA to prevent spontaneous lupus-like inflammatory disease. Proc Natl Acad Sci U S A. 2015 Apr 21; 112(16):5117-22. PMID: 25848017.
      Citations:    
    10. Davis RR, Shaban NM, Perrino FW, Hollis T. Crystal structure of RNA-DNA duplex provides insight into conformational changes induced by RNase H binding. . 2015; 14(4):668-73. PMID: 25664393.
      Citations:    
    11. Fye JM, Coffin SR, Orebaugh CD, Hollis T, Perrino FW. The Arg-62 residues of the TREX1 exonuclease act across the dimer interface contributing to catalysis in the opposing protomers. J Biol Chem. 2014 Apr 18; 289(16):11556-11565. PMID: 24616097.
      Citations:    
    12. Orebaugh CD, Fye JM, Harvey S, Hollis T, Wilkinson JC, Perrino FW. The TREX1 C-terminal region controls cellular localization through ubiquitination. J Biol Chem. 2013 Oct 04; 288(40):28881-92. PMID: 23979357.
      Citations:    
    13. Rice GI, Reijns MA, Coffin SR, Forte GM, Anderson BH, Szynkiewicz M, Gornall H, Gent D, Leitch A, Botella MP, Fazzi E, Gener B, Lagae L, Olivieri I, Orcesi S, Swoboda KJ, Perrino FW, Jackson AP, Crow YJ. Synonymous mutations in RNASEH2A create cryptic splice sites impairing RNase H2 enzyme function in Aicardi-Goutières syndrome. Hum Mutat. 2013 Aug; 34(8):1066-70. PMID: 23592335.
      Citations:    
    14. Bailey SL, Harvey S, Perrino FW, Hollis T. Defects in DNA degradation revealed in crystal structures of TREX1 exonuclease mutations linked to autoimmune disease. DNA Repair (Amst). 2012 Jan 02; 11(1):65-73. PMID: 22071149.
      Citations:    
    15. Powell RD, Holland PJ, Hollis T, Perrino FW. Aicardi-Goutieres syndrome gene and HIV-1 restriction factor SAMHD1 is a dGTP-regulated deoxynucleotide triphosphohydrolase. J Biol Chem. 2011 Dec 23; 286(51):43596-43600. PMID: 22069334.
      Citations:    
    16. Orebaugh CD, Fye JM, Harvey S, Hollis T, Perrino FW. The TREX1 exonuclease R114H mutation in Aicardi-Goutières syndrome and lupus reveals dimeric structure requirements for DNA degradation activity. J Biol Chem. 2011 Nov 18; 286(46):40246-54. PMID: 21937424.
      Citations:    
    17. Fye JM, Orebaugh CD, Coffin SR, Hollis T, Perrino FW. Dominant mutation of the TREX1 exonuclease gene in lupus and Aicardi-Goutieres syndrome. J Biol Chem. 2011 Sep 16; 286(37):32373-82. PMID: 21808053.
      Citations:    
    18. Coffin SR, Hollis T, Perrino FW. Functional consequences of the RNase H2A subunit mutations that cause Aicardi-Goutieres syndrome. J Biol Chem. 2011 May 13; 286(19):16984-91. PMID: 21454563.
      Citations:    
    19. Namjou B, Kothari PH, Kelly JA, Glenn SB, Ojwang JO, Adler A, Alarcón-Riquelme ME, Gallant CJ, Boackle SA, Criswell LA, Kimberly RP, Brown E, Edberg J, Stevens AM, Jacob CO, Tsao BP, Gilkeson GS, Kamen DL, Merrill JT, Petri M, Goldman RR, Vila LM, Anaya JM, Niewold TB, Martin J, Pons-Estel BA, Sabio JM, Callejas JL, Vyse TJ, Bae SC, Perrino FW, Freedman BI, Scofield RH, Moser KL, Gaffney PM, James JA, Langefeld CD, Kaufman KM, Harley JB, Atkinson JP. Evaluation of the TREX1 gene in a large multi-ancestral lupus cohort. Genes Immun. 2011 Jun; 12(4):270-9. PMID: 21270825.
      Citations:    
    20. Pence MG, Blans P, Zink CN, Fishbein JC, Perrino FW. Bypass of N²-ethylguanine by human DNA polymerase ?. DNA Repair (Amst). 2011 Jan 02; 10(1):56-64. PMID: 20952260.
      Citations:    
    21. Shaban NM, Harvey S, Perrino FW, Hollis T. The structure of the mammalian RNase H2 complex provides insight into RNA.NA hybrid processing to prevent immune dysfunction. J Biol Chem. 2010 Feb 05; 285(6):3617-3624. PMID: 19923215.
      Citations:    
    22. de Silva U, Perrino FW, Hollis T. DNA binding induces active site conformational change in the human TREX2 3'-exonuclease. Nucleic Acids Res. 2009 Apr; 37(7):2411-7. PMID: 19321497.
      Citations:    
    23. Casteel DE, Zhuang S, Zeng Y, Perrino FW, Boss GR, Goulian M, Pilz RB. A DNA polymerase-{alpha}{middle dot}primase cofactor with homology to replication protein A-32 regulates DNA replication in mammalian cells. J Biol Chem. 2009 Feb 27; 284(9):5807-18. PMID: 19119139.
      Citations:    
    24. Perrino FW, Harvey S, Shaban NM, Hollis T. RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases. J Mol Med (Berl). 2009 Jan; 87(1):25-30. PMID: 19034401.
      Citations:    
    25. Pence MG, Blans P, Zink CN, Hollis T, Fishbein JC, Perrino FW. Lesion bypass of N2-ethylguanine by human DNA polymerase iota. J Biol Chem. 2009 Jan 16; 284(3):1732-40. PMID: 18984581.
      Citations:    
    26. Lehtinen DA, Harvey S, Mulcahy MJ, Hollis T, Perrino FW. The TREX1 double-stranded DNA degradation activity is defective in dominant mutations associated with autoimmune disease. J Biol Chem. 2008 Nov 14; 283(46):31649-56. PMID: 18805785.
      Citations:    
    27. Perrino FW, de Silva U, Harvey S, Pryor EE, Cole DW, Hollis T. Cooperative DNA binding and communication across the dimer interface in the TREX2 3' --> 5'-exonuclease. J Biol Chem. 2008 Aug 01; 283(31):21441-52. PMID: 18534978.
      Citations:    
    28. Lee-Kirsch MA, Gong M, Chowdhury D, Senenko L, Engel K, Lee YA, de Silva U, Bailey SL, Witte T, Vyse TJ, Kere J, Pfeiffer C, Harvey S, Wong A, Koskenmies S, Hummel O, Rohde K, Schmidt RE, Dominiczak AF, Gahr M, Hollis T, Perrino FW, Lieberman J, Hübner N. Mutations in the gene encoding the 3'-5' DNA exonuclease TREX1 are associated with systemic lupus erythematosus. Nat Genet. 2007 Sep; 39(9):1065-7. PMID: 17660818.
      Citations:    
    29. Lee-Kirsch MA, Chowdhury D, Harvey S, Gong M, Senenko L, Engel K, Pfeiffer C, Hollis T, Gahr M, Perrino FW, Lieberman J, Hubner N. A mutation in TREX1 that impairs susceptibility to granzyme A-mediated cell death underlies familial chilblain lupus. J Mol Med (Berl). 2007 May; 85(5):531-7. PMID: 17440703.
      Citations:    
    30. Rice G, Newman WG, Dean J, Patrick T, Parmar R, Flintoff K, Robins P, Harvey S, Hollis T, O'Hara A, Herrick AL, Bowden AP, Perrino FW, Lindahl T, Barnes DE, Crow YJ. Heterozygous mutations in TREX1 cause familial chilblain lupus and dominant Aicardi-Goutieres syndrome. Am J Hum Genet. 2007 Apr; 80(4):811-5. PMID: 17357087.
      Citations:    
    31. de Silva U, Choudhury S, Bailey SL, Harvey S, Perrino FW, Hollis T. The crystal structure of TREX1 explains the 3' nucleotide specificity and reveals a polyproline II helix for protein partnering. J Biol Chem. 2007 Apr 06; 282(14):10537-43. PMID: 17293595.
      Citations:    
    32. Harrigan JA, Fan J, Momand J, Perrino FW, Bohr VA, Wilson DM. WRN exonuclease activity is blocked by DNA termini harboring 3' obstructive groups. Mech Ageing Dev. 2007 Mar; 128(3):259-66. PMID: 17224176.
      Citations:    
    33. Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC. Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex. J Biol Chem. 2006 Dec 15; 281(50):38466-71. PMID: 16973612.
      Citations:    
    34. Chowdhury D, Beresford PJ, Zhu P, Zhang D, Sung JS, Demple B, Perrino FW, Lieberman J. The exonuclease TREX1 is in the SET complex and acts in concert with NM23-H1 to degrade DNA during granzyme A-mediated cell death. Mol Cell. 2006 Jul 07; 23(1):133-42. PMID: 16818237.
      Citations:    
    35. Upton DC, Wang X, Blans P, Perrino FW, Fishbein JC, Akman SA. Replication of N2-ethyldeoxyguanosine DNA adducts in the human embryonic kidney cell line 293. Chem Res Toxicol. 2006 Jul; 19(7):960-7. PMID: 16841965.
      Citations:    
    36. Upton DC, Wang X, Blans P, Perrino FW, Fishbein JC, Akman SA. Mutagenesis by exocyclic alkylamino purine adducts in Escherichia coli. Mutat Res. 2006 Jul 25; 599(1-2):1-10. PMID: 16488449.
      Citations:    
    37. Perrino FW, Harvey S, Blans P, Gelhaus S, Lacourse WR, Fishbein JC. Polymerization past the N2-isopropylguanine and the N6-isopropyladenine DNA lesions with the translesion synthesis DNA polymerases eta and iota and the replicative DNA polymerase alpha. Chem Res Toxicol. 2005 Sep; 18(9):1451-61. PMID: 16167838.
      Citations:    
    38. Perrino FW, Harvey S, McMillin S, Hollis T. The human TREX2 3' -> 5'-exonuclease structure suggests a mechanism for efficient nonprocessive DNA catalysis. J Biol Chem. 2005 Apr 15; 280(15):15212-8. PMID: 15661738.
      Citations:    
    39. Lehtinen DA, Perrino FW. Dysfunctional proofreading in the Escherichia coli DNA polymerase III core. Biochem J. 2004 Dec 01; 384(Pt 2):337-48. PMID: 15352874.
      Citations:    
    40. Perrino FW, Krol A, Harvey S, Zheng SL, Horita DA, Hollis T, Meyers DA, Isaacs WB, Xu J. Sequence variants in the 3'-->5' deoxyribonuclease TREX2: identification in a genetic screen and effects on catalysis by the recombinant proteins. Adv Enzyme Regul. 2004; 44:37-49. PMID: 15581481.
      Citations:    
    41. Perrino FW, Blans P, Harvey S, Gelhaus SL, McGrath C, Akman SA, Jenkins GS, LaCourse WR, Fishbein JC. The N2-ethylguanine and the O6-ethyl- and O6-methylguanine lesions in DNA: contrasting responses from the "bypass" DNA polymerase eta and the replicative DNA polymerase alpha. Chem Res Toxicol. 2003 Dec; 16(12):1616-23. PMID: 14680376.
      Citations:    
    42. DeRose EF, Darden T, Harvey S, Gabel S, Perrino FW, Schaaper RM, London RE. Elucidation of the epsilon-theta subunit interface of Escherichia coli DNA polymerase III by NMR spectroscopy. Biochemistry. 2003 Apr 08; 42(13):3635-44. PMID: 12667053.
      Citations:    
    43. DeRose EF, Li D, Darden T, Harvey S, Perrino FW, Schaaper RM, London RE. Model for the catalytic domain of the proofreading epsilon subunit of Escherichia coli DNA polymerase III based on NMR structural data. Biochemistry. 2002 Jan 08; 41(1):94-110. PMID: 11772007.
      Citations:    
    Perrino's Networks
    Click the
    Explore
    buttons for more information and interactive visualizations!
    Concepts (231)
    Explore
    _
    Co-Authors (6)
    Explore
    _
    Similar People (59)
    Explore
    _
    Same Department Expand Description
    Explore
    _
    Physical Neighbors
    _